ea hy926 human endothelial cells  (ATCC)

Journal: Molecular Medicine Reports

Article Title: PTUPB, a soluble epoxide hydrolase/cyclooxygenase-2 dual inhibitor, reduces endothelial-to-mesenchymal transition and improves doxorubicin-induced vascular and cardiac toxicity

doi: 10.3892/mmr.2026.13810

Figure Lengend Snippet: PTUPB decreases DOX-induced sEH and COX-2 expression in human endothelial cells. (A) Basal gene expression levels of sEH and COX-2 in endothelial cells. EA.hy926 cells were treated with 0.5 µM, 1 µM and 2 µM DOX for 24H. Gene expression levels of (B) sEH and (C) COX-2 in DOX-treated cells. Viability of EA.hy926 cells treated with either (D) PTUPB alone or (E) in combination with DOX. EA.hy926 cells were treated with 2 µM DOX with or without 1 µM PTUPB for 24 h. Gene expression levels of (F) sEH and (G) COX-2. (H) Activity level of sEH. One-way ANOVA followed by Tukey's multiple comparison tests was performed. + P<0.05 vs. control, *P<0.05 vs. DOX). sEH, soluble epoxide hydrolase; COX-2, cyclooxygenase-2; DOX, doxorubicin; PTUPB, 4-[5-phenyl-3-[3-[[[[4-(trifluoromethyl)phenyl]amino]carbonyl]amino]propyl]-1H-pyrazol-1-yl]-benzenesulfonamide; DHET, dihydroxyeicosatrienoic acid; Con, control.

Article Snippet: MDA-MB-231 cells (cat. no. HTB-26; American Type Culture Collection) and EA.hy926 human endothelial cells (cat. no. CRL-2922; American Type Culture Collection) were cultured in 75-cm 2 tissue-culture flasks that contained Dulbecco's Modified Eagle Medium F-12 Nutrient Mixture supplemented with 10% w/v fetal bovine serum and 1% w/v Antibiotic-Antimycotic (all Gibco; Thermo Fisher Scientific, Inc.) in a humid environment at 37°C with 5% CO 2 .

Techniques: Expressing, Gene Expression, Activity Assay, Comparison, Control

Journal: Molecular Medicine Reports

Article Title: PTUPB, a soluble epoxide hydrolase/cyclooxygenase-2 dual inhibitor, reduces endothelial-to-mesenchymal transition and improves doxorubicin-induced vascular and cardiac toxicity

doi: 10.3892/mmr.2026.13810

Figure Lengend Snippet: Inhibition of soluble epoxide hydrolase and cyclooxygenase-2 using PTUPB attenuates DOX-induced endothelial-to-mesenchymal transition. EA.hy926 cells were treated with 2 µM DOX with or without 1 µM PTUPB for 24 h. Gene expression levels of mesenchymal markers (A) ASMA, (B) SMA22, (C) VIM, (D) CDH2, (E) TGF-β, (F) SNAI1 and (G) SNAI2, and (H) endothelial marker CD31. (I) Ratio of mesenchymal to endothelial cells in cells treated with DOX with or without 1 µM PTUPB was measured based on morphological images. (J) Representative morphological images of EA.hy926 cells incubated with DOX with or without 1 µM PTUPB for 24 h at a magnification of ×10 (scale bar, 100 µm). The light red arrow indicates mesenchymal cells (long, spindle-like cells). The dark red arrow indicates endothelial cells (cobblestone monolayer-like cells). One-way ANOVA followed by Tukey's multiple comparison test was performed to determine the significant differences between groups ( + P<0.05 vs. control, *P<0.05 vs. DOX). PTUPB, 4-[5-phenyl-3-[3-[[[[4-(trifluoromethyl)phenyl]amino]carbonyl]amino]propyl]-1H-pyrazol-1-yl]-benzenesulfonamide; DOX, doxorubicin; ASMA, smooth muscle actin α2; SMA22, smooth muscle protein 22α; VIM, vimentin; CDH2, cadherin-2; SNAI1, snail family transcriptional repressor 1; SNAI2, snail family transcriptional repressor 2.

Article Snippet: MDA-MB-231 cells (cat. no. HTB-26; American Type Culture Collection) and EA.hy926 human endothelial cells (cat. no. CRL-2922; American Type Culture Collection) were cultured in 75-cm 2 tissue-culture flasks that contained Dulbecco's Modified Eagle Medium F-12 Nutrient Mixture supplemented with 10% w/v fetal bovine serum and 1% w/v Antibiotic-Antimycotic (all Gibco; Thermo Fisher Scientific, Inc.) in a humid environment at 37°C with 5% CO 2 .

Techniques: Inhibition, Gene Expression, Marker, Incubation, Comparison, Control